Effects of FMN on antioxidant defense in LO2 cells.

<p>Human non-tumor hepatic LO2 cells were maintained in DMEM medium supplemented with 10% FBS and incubated in a humidified incubator at 37°C in 5% CO2. The cell was initiated by replacing old media with a serum-free medium supplemented with or without FMN (20, 40 μM) for 6 h. Then, the cells were further incubated with APAP 10 mM for another 24 h. Cells collected were washed twice with PBS and used for GSH or cell viability analyses. (*) the APAP exposure <i>vs</i> control; (#) APAP+ 20 μM or APAP+ 40 μM <i>vs</i> the APAP exposure. Values represent the means ± SE; <i>n</i> = 4. #, <i>p</i> < 0.05; *** or ###, <i>p</i> < 0.001. (A) Effects of FMN on Nrf2 expression in LO2 cells. (B) Effects of FMN on mRNA expression of antioxidant enzymes in LO2 cells, as determined by quantitative real-time RT-PCR. (C) Effect of FMN on GSH levels in LO2 with 10 mM APAP exposure. (D) Effect of FMN on the content of ROS in LO2 with 10 mM APAP exposure. (E) Effect of FMN on cell viability in LO2 with 10 mM APAP exposure.