Metabolic capacity of HeLa compared to A549.

<p>Fatty acid profiles were determined by GC-FID analysis of cellular lipid extracts and are expressed as % of total FAME. Data represent means ± SD.</p>a<p>Cells were incubated without (−) or with (+) 33 µM of C18∶2<i>n</i>−6 and C18∶3<i>n</i>−3, respectively, for 24 h. Both are substrates for FADS2.</p>b<p>Cells were incubated without (−) or with (+) 33 µM of the FADS2 products C18∶3<i>n</i>−6 and C18∶4<i>n</i>−3 for 24 h.</p>c<p>Considered Δ8-desaturation products.</p>d<p>Synthesis requires upstream a second step of <i>FADS2</i>-encoded activity.</p>e<p>After 72 h, significant increase in A459 to 2.7±0.2 when incubated with C18∶3<i>n</i>−3 (FA treatment×cell line: p = .002; see also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115610#pone-0115610-g001" target="_blank">Figure 1B</a>, peak 14) and to 2.8±0.1 when incubated with C18∶4<i>n</i>−3 (FA treatment×cell line: p = .011).</p><p>Metabolic capacity of HeLa compared to A549.