Product-to-substrate ratios as estimates of desaturase activity in HeLa and A549.
- Anke Jaudszus (422810)
- Christian Degen (680154)
- Stephan W. Barth (680155)
- Martin Klempt (680156)
- Wiebke Schlörmann (680157)
- Alexander Roth (680158)
- Carsten Rohrer (680159)
- Helga Sauerwein (333649)
- Konrad Sachse (44348)
- Gerhard Jahreis (680160)
Publication date
December 2014
DOIAbstract
<p>FADS2 ratio: (C18∶3<i>n</i>−6+C20∶3<i>n</i>−6)/C18∶2<i>n</i>−6 and (C18∶4<i>n</i>−3+C20∶4<i>n</i>−3)/C18∶3<i>n</i>−3 after 24 h (with the second FADS2-dependent step not considered), FADS1 ratio: C20∶4<i>n</i>−6/C20∶3<i>n</i>−6 and C20∶5<i>n</i>−3/C20∶4<i>n</i>−3 after 24 h. Increase in FADS2 ratios is significantly different from HeLa (***p≤.001).</p
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