Partial chromatograms of cellular lipids of HeLa and A549.

<p>Cells were incubated without (DMSO-treated control) or with the indicated fatty acid (33 µM) for (<b>A</b>) 24 h or (<b>B</b>) for 72 h. 1–C18∶0, 2–C18∶1<i>c</i>9, 3–C18∶1<i>c</i>11, 4–C18∶2<i>n</i>−6, 5–C18∶3<i>n</i>−6, 6–C20∶2<i>n</i>−6, 7–C20∶3<i>n</i>−6, 8–C20∶4<i>n</i>−6, 9–C22∶4<i>n</i>−6, 10–C22∶5<i>n</i>−6, 11–C18∶3<i>n</i>−3, 12–C20∶5<i>n</i>−3, 13–C22∶5<i>n</i>−3, 14–C22∶6<i>n</i>−3. <b>A:</b> C20∶2<i>n</i>−6 (peak 6) was the only metabolite identified in HeLa following incubation with C18∶2<i>n</i>−6. In contrast, all detectable elongated and desaturated products of C18∶2<i>n</i>−6 increased in A549 (filled-headed arrows). When C18∶3<i>n</i>−6 was the supplement, the respective metabolites increased in HeLa, too (broken-lined arrows). <b>B:</b> As a product of C18∶3<i>n</i>−3, C22∶6<i>n</i>−3 (peak 14) increased in A549 after 72 h.