Time-dependent cytochrome c release, caspase-3/-7 activity and DNA fragmentation in hepatocytes treated with IL-1β and FasL.

<p>Primary hepatocytes were treated with FasL or IL-1β + FasL for the indicated times before performing cytochrome c release by ELISA (A), a fluorogenic DEVDase caspase assay (B), or a cell death DNA fragmentation assay (C). Values for the DNA fragmentation assay are referred to the untreated control. Five independent experiments ± s.d. are displayed except for the incubation time 12+3 h (n = 4) (*p<0.05, ***p<0.001 IL-1β + FasL versus FasL treated cells at the corresponding time point).