Fluorescence-based cytotoxicity assay and chromatin condensation analysis.
- Subhasis Das (302747)
- Kaushik Kumar Dey (302748)
- Goutam Dey (302749)
- Ipsita Pal (232454)
- Abhijit Majumder (302750)
- Sujata MaitiChoudhury (302751)
- Subhas C. kundu (302752)
- Mahitosh Mandal (302755)
Publication date
February 2013
DOIAbstract
<p>(A) Fluorescence-based cytotoxicity assay (using the LIVE/DEAD Viability/Cytotoxicity kit) of A431 and Hep2cells treated with TQ or DG for 24 or 48 hours. A significantly higher percentage of apoptotic cells (live (green)/dead (red)) are observed in the treated groups than in controls. (B) Fluorescence microscopic observation of A431 and Hep2 treated with IC<sub>50</sub> of TQ and DG for 24 or 48 hours and stained with DAPI to detect chromatin condensation.</p
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