FtsA effect on GTPase activity of FtsZ from <i>Deinococcus radiodurans</i> as well as <i>Escherichia coli</i>.

<p>Purified recombinant Dr-FtsZ and Ec-FtsZ were incubated in different combinations with GTP, ATP, Dr-FtsA (Dr) and Ec-FtsA (Ec) and the release of inorganic phosphate (Pi) was monitored (<b>A, B</b>). Similarly, Dr-FtsA (FtsA), Dr-FtsZ (FtsZ), ATP and [α 32P] GTP were incubated in different combinations and conversion of [α32P]-GTP substrate ([³²P]α GTP) into [α32P] GDP product ([³²P]α GDP) was monitored on TLC and autoradiogram was developed (<b>C</b>). Levels of both substrate (GTP) and product (GDP) were determined densitometrically for samples numbered as 1–6 as shown in panel C and plotted (<b>D</b>).