<i>In vitro</i> interaction of FtsZ with FtsA.

<p>Increasing concentration (1–10 µg) of Dr-FtsA and Ec-FtsA were incubated in the absence (<b>A</b>) and presence of ATP (<b>B</b>) for 10 min and blotted on nitrocellulose support (Protein). Blots were incubated separately with 1 µg/ml of purified recombinant Dr-FtsZ and Ec-FtsZ overnight at 4°C as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115918#s2" target="_blank">methods</a>, washed gently and hybridized with antibodies against Dr-FtsZ (Anti-DrFtsZ) and Ec-FtsZ (Anti-EcFtsZ), respectively. Signals were detected using NBT/BCIP system for alkaline phosphatase. BSA was taken as a control and processed in parallel. Data shown are representatives of the reproducible experiments repeated three times.