Introduction Nucleoside diphosphate kinase (NDK), conserved across bacteria to humans, synthesises NTP from NDP and ATP. The eukaryotic homologue, the NDPK, uses ATP to phosphorylate the tubulin-bound GDP to GTP for tubulin polymerisation. The bacterial cytokinetic protein FtsZ, which is the tubulin homologue, also uses GTP for polymerisation. Therefore, we examined whether NDK can interact with FtsZ to convert FtsZ-bound GDP and/or free GDP to GTP to trigger FtsZ polymerisation. Methods Recombinant and native NDK and FtsZ proteins of Mycobacterium smegmatis and Mycobacterium tuberculosis were used as the experimental samples. FtsZ polymersation was monitored using 90 degrees light scattering and FtsZ polymer pelleting assays. The gamma 32P...
Cytokinesis in bacteria depends upon the contractile Z ring, which is composed of dynamic polymers o...
AbstractThe prokaryotic tubulin homologue FtsZ polymerizes in vitro in a nucleotide dependent fashio...
The primary structure and function of nucleoside diphosphate kinase (NDK), a substrate non-specific ...
Introduction Nucleoside diphosphate kinase (NDK), conserved across bacteria to humans, synthesises N...
AbstractStable, more than 98% nucleotide-free apo-FtsZ was prepared from purified Methanococcus jann...
FtsZ, a tubulin homologue, forms a cytokinetic ring at the site of cell division in prokaryotes. The...
FtsZ, a tubulin homologue, forms a cytokinetic ring at the site of cell division in prokaryotes. The...
To understand the polymerization dynamics of FtsZ, a bacterial cell division protein similar to tubu...
6 páginas, 4 figuras, 2 tablas -- PAGS nros. 43-48Stable, more than 98% nucleotide-free apo-FtsZ was...
AbstractBacteria and archaea usually divide symmetrically by formation of a septum in the middle of ...
The essential prokaryotic cell division protein FtsZ is a tubulin homologue that forms a ring at the...
SummaryThe cytoskeletal proteins, FtsZ and tubulin, play a pivotal role in prokaryotic cell division...
Essential cell division protein FtsZ forms the bacterial cytokinetic ring and is a target for new an...
International audienceThe cytoskeletal proteins, FtsZ and tubulin, play a pivotal role in prokaryoti...
The cytoskeletal proteins, FtsZ and tubulin, play a pivotal role in prokaryotic cell division and eu...
Cytokinesis in bacteria depends upon the contractile Z ring, which is composed of dynamic polymers o...
AbstractThe prokaryotic tubulin homologue FtsZ polymerizes in vitro in a nucleotide dependent fashio...
The primary structure and function of nucleoside diphosphate kinase (NDK), a substrate non-specific ...
Introduction Nucleoside diphosphate kinase (NDK), conserved across bacteria to humans, synthesises N...
AbstractStable, more than 98% nucleotide-free apo-FtsZ was prepared from purified Methanococcus jann...
FtsZ, a tubulin homologue, forms a cytokinetic ring at the site of cell division in prokaryotes. The...
FtsZ, a tubulin homologue, forms a cytokinetic ring at the site of cell division in prokaryotes. The...
To understand the polymerization dynamics of FtsZ, a bacterial cell division protein similar to tubu...
6 páginas, 4 figuras, 2 tablas -- PAGS nros. 43-48Stable, more than 98% nucleotide-free apo-FtsZ was...
AbstractBacteria and archaea usually divide symmetrically by formation of a septum in the middle of ...
The essential prokaryotic cell division protein FtsZ is a tubulin homologue that forms a ring at the...
SummaryThe cytoskeletal proteins, FtsZ and tubulin, play a pivotal role in prokaryotic cell division...
Essential cell division protein FtsZ forms the bacterial cytokinetic ring and is a target for new an...
International audienceThe cytoskeletal proteins, FtsZ and tubulin, play a pivotal role in prokaryoti...
The cytoskeletal proteins, FtsZ and tubulin, play a pivotal role in prokaryotic cell division and eu...
Cytokinesis in bacteria depends upon the contractile Z ring, which is composed of dynamic polymers o...
AbstractThe prokaryotic tubulin homologue FtsZ polymerizes in vitro in a nucleotide dependent fashio...
The primary structure and function of nucleoside diphosphate kinase (NDK), a substrate non-specific ...