Bacterial enumeration at moribund disease for respiratory melioidosis models.

<p>Groups of 5 female albino C57BL/6J mice were infected by either the i.n. (10^5.1 CFU) or IMIT (10^4.6 CFU) routes of infection and euthanized at moribund disease endpoints. Bacteria were enumerated from tissues homogenized in 1 ml PBS, from a 1 ml PBS BAL collection, or from cardiac-drawn blood. Bacterial burden was calculated as CFU/tissue (lung, liver, and spleen) or bacteria per ml of body fluid (BAL and blood). Significant differences between log transformed data were evaluated by 2-way ANOVA with Bonferroni multiple comparisons (n.s., not significant; *, p<0.05; **, p<0.01; ****, p<0.0001).