Median lethal dose determination of WT <i>B</i>. <i>mallei</i> and recombinant <i>bpaB</i> KO strains.

<p>Mice were inoculated intratracheally using a Microsprayer device to aerosolize the indicated number of bacterial CFU directly into the lungs (n = 5 mice/dose). Animals were then monitored daily for clinical signs of illness and morbidity. <u>Panel A</u>: Survival curves. <u>Panel B</u>: Calculated LD₅₀ values. <u>Panels C and D</u>: Tissues were collected from mice that survived challenge with 10² CFU, homogenized, diluted, and spread on agar plates to determine bacterial loads. Symbols show data for individual animals.