Optimization of Molar Ratio NF-κB-DNA.

<p><b>a)</b> Agarose gel showing the effect of NF-κB on the palindromic DNA-recognition target. NF-κB-DNA molar ratio per Lane: 1 (253:1), 2 (316:1), 3 (379:1), 4 (443:1), 5 (506:1), 6 (569:1), B1 (Blank with HEPES Buffer) and B2 (Blank with nuclease-free water). <b>b)</b> (′) Standard addition curve for each molar ratio of NF-κB:DNA target (as lane number), y = -0.0199x + 0.9854, R²; = 0.98911; (<b>X</b>) Standard addition curve for each molar ratio of Bovine serum albumin:DNA target (as lane number).