Optimization of the UP-M-PCR.

<p>Lane A, B, C, D, E, amplicon fragments by UP (500 nmol L⁻¹) and compound specific primer hpt-839, nptII-508, pat-262, bar-226 and sps-110 at a series concentrations of 500 nmol L⁻¹, 50 nmol L⁻¹, 25 nmol L⁻¹, 5 nmol L⁻¹, 0.5 nmol L⁻¹; lane F1, amplicon fragments by UP at 500 nmol L⁻¹ and all compound specific primers at 25 nmol L⁻¹; lane F2, amplicon fragments by UP at 500 nmol L⁻¹ and all compound specific primers at the optimized concentration; lane G1,G2,G3, amplicon fragments by all primers at the optimized concentration with <i>TaKaRa Taq</i>™, Phire™ Hot Start DNA polymerase, iProof™ High-Fidelity DNA polymerase; lane H1, amplicon fragments by all primers at the optimized concentration with Phire™ Hot Start DNA polymerase under the common amplification conditions; lane H2, amplicon fragments by all primers at the optimized concentration with Phire™ Hot Start DNA polymerase under the optimized amplification conditions; lane M, 100 bp DNA Marker.