Fkh2 is phosphorylated at serine 533 by Cbk1-Mob2.

<p>A) Autoradiograms from 2D gels and quantitative intensity profiles of the indicated strains at the indicated times and culture conditions. The grey dashed line represents the wild type Fkh2-YFP profile grown in the corresponding condition as shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004630#ppat.1004630.g001" target="_blank">Fig. 1</a>. B) <i>In vitro</i> kinase assay with Mob2-HA purified from a 40 min hyphal culture and <i>E. coli</i> expressed Fkh2 C-terminal fragment used in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004630#ppat.1004630.g002" target="_blank">Fig. 2C</a>. A BWP17 mock lysate controls for the possibility of a co-precipitating kinase from the αHA IP. C) Morphology of <i>fkh2</i> S533A mutants. <i>fkh2/FKH2</i> and <i>fkh2/fkh2(S533A)</i>. Cells were re-inoculated from stationary phase cultures into either yeast or hyphal growth conditions, grown for 6 h and then fixed with 1.5% formaldehyde. Hyphal cells were pepsin treated to remove clumps before imaging at x100 magnification. Scale bars represent 10 μm.