Phosphorylation of Fkh2 affects the long-term maintenance of hyphal growth.

<p>A) Phenotypes of Fkh2 phosphorylation mutants of the indicated genotypes. Left: grown as hyphae for 6 h in GMM with 10% FCS, fixed with 1.5% formaldehyde and pepsin treated. White arrows indicate branching and black arrows indicate constrictions. Right: yeast cells were re-inoculated into GMM pH 4.0 for 4hrs before formaldehyde fixation. Scale bars are equal to 10 μm. B) and C). Quantitation (n = 50) of the hyphal phenotypes was carried out to quantitate hyphal branching (B), and constrictions within a hypha (C). For Figs. B and C, a Z-test was used to compare the proportion of cells displaying the phenotype in the mutants with the wild type. * z < 0.05, ** z < 0.01. D) Long-term effects on hyphal growth observed on solid Spider medium. An overnight culture was diluted to OD₆₀₀ = 1.0 and then serially diluted 10 fold as shown, with 1 μl of each dilution being spotted and the plates left at 37°C for five days. The extent of invasion was observed through washing off the surface colony using deionised water.