p53 DNA binding domain sequence alignment of Homo sapiens, Mus musculus, Xenopus laevis, Danio rerio, Drosophila melanogaster and Caenorhabditis elegans.

<p>Sequences were aligned using ClustalW tool [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116177#pone.0116177.ref074" target="_blank">74</a>] and the alignment was visualized using Jalview (<a href="http://www.jalview.org" target="_blank">http://www.jalview.org</a>) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116177#pone.0116177.ref039" target="_blank">39</a>]. The three shades of blue highlight different percentage agreement, respectively from darker to lighter in this order: >80%, >60%, >40%. A percentage agreement ≤40% is not highlighted. The alignment shows the conservation of human functional residues involved in DNA-protein interaction (solid box), zinc-binding (dashed box), DBD stability (asterisk, *), protein-protein interface (empty circle, #) and DBD thermostability (hash mark, #). Human zinc-binding and DNA-binding residue positions are also typed. On the top of the alignment, human p53 Loop, Sheet, Helix motifs (L, dashed lines; S, left-right double arrows; H, two-dimensional arrows) are also presented. Conservation and consensus graphs are shown. Conservation is visualized as a histogram with the relative score for each column. Conserved columns are indicated with an asterisk, and columns with mutations, where all properties are conserved, are marked with a plus. Consensus is displayed as the percentage of the modal residue per column. The plus symbol is used instead of displaying multiple characters in a single character space. A consensus logo is also generated and the scale of the letter is in agreement with the conservation of the residues.