Amino-acid sequence alignment, domain organisation, gene organisation and phylogeny of DC-SCRIPT from <i>L</i>. <i>calcarifer</i>.

<p>(a) Amino acid sequences comparison between barramundi (<i>L</i>. <i>calcarifer</i>) and human (<i>H</i>. <i>sapiens</i>) DC-SCRIPT, aligned on the NCBI website (<a href="http://www.ncbi.nlm.nih.gov/" target="_blank">http://www.ncbi.nlm.nih.gov</a>) by protein BLAST. The green box represents the nuclear targeting sequence consensus (KRK*) as per Chelsky, Ralph [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132687#pone.0132687.ref051" target="_blank">51</a>]. The black boxes represent a proline rich region and an acidic region at the N- and C-ends respectively. The 11 orange boxes represent the 11 zinc finger sequences as identified by Triantis, Trancikova [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132687#pone.0132687.ref016" target="_blank">16</a>]. (b) Domain organisation of DC-SCRIPT from different species predicted using SMART online software. Blue rectangles represent C2H2 zinc fingers, pink rectangles represent low complexity regions and the green rectangle represents a coiled coil region. (c) Gene organisation of barramundi DC-SCRIPT. The orange boxes represent the exons. Numbers are representative of the number of base pairs, totalling 5337 bp between stop codons. (d) Phylogenetic tree is representative of a Maximum Likelihood analysis performed on CLUSTALW aligned sequences (MEGA 5.2.2).