Morphology change in pyramidal and granule cells.

<p>(A-B) Typical morphology of pyramidal and granule cells at DIV 7 (A) and DIV 16 (B) of cultures prepared from P0 mice. Cells were visualized by GFP transfection. Cell types were identified by Math2 and Prox1 immunostaining (magenta). The principal dendrite and axon were indicated by white arrowhead and asterisk, respectively. Scale bar: 40 μm. (C-E) Quantification of total dendritic length (C), the number of branch terminals (D) and primary dendrites (E) during dendrite development. n = 20. Student's t-test: *P<0.05, **P<0.03, ***P<0.001.