The morphology of the apical dendritic tree of granule cells with hilar basal dendrites.

<p>(A, B) Alexa-filled dentate granule cells were reconstructed in confocal image stacks using Neuronstudio software. 2D-projected image stacks (left) and the corresponding reconstructed skeletons (right) are shown for a granule cell without (A) and with a hBD (B). Scale bar: 50 µm. (C–E) No significant difference in total dendritic branch length (TDBL), number of segments per branch order or mean length per branch order was observed between granule cells with and without basal dendrites (n = 21 GCs without hBDs and n = 21 GCs with hBDs; in 12 cultures). (F, G) The Sholl-analysis of the two GC populations showed a comparable complexity of the dendritic trees (distance of circles/spheres in F, 25 µm).