Altered dendritic morphology in IGZ dentate granule cells from GluN2A^−/− mice.

<p>(<b><i>A, D</i></b>) Total dendritic length was significantly decreased in GluN2A^−/− inner granular zone (IGZ) cells (<b><i>A</i></b>) and unaltered in GluN2A^−/− outer granular zone (OGZ) cells (<b><i>D</i></b>). <b><i>B, E,</i></b> Sholl analysis of IGZ (<b><i>B</i></b>) and OGZ (<b><i>E</i></b>) cells. A repeated measures ANOVA revealed a main effect of genotype in IGZ cells (<b><i>B</i></b>), with GluN2A^−/− IGZ cells having a lower number of dendrites per ring intersection from 80–160 µm from soma. No effect of genotype was seen in OGZ cells (<b><i>E</i></b>). (<b><i>C, F</i></b>) Branch order analysis of IGZ (<b><i>C</i></b>) and OGZ (<b><i>F</i></b>) cells. A repeated measures ANOVA revealed a main effect of genotype in IGZ cells (<b><i>C</i></b>), showing a significantly less number of third-order branches in GluN2A^−/− IGZ cells. OGZ cells did not show a main effect of genotype (<b><i>F</i></b>). For all graphs, WT: black; GluN2A^−/−: white. Data is represented as means ± SEM. * denotes statistical significant difference (<i>p</i><0.05).