Effect of GS12021 treatment on AMPKα activation and SIRT1 expression in RAW 264.7 cells.

<p><b>(A)</b> Levels of phosphorylated AMPKα and total AMPKα were measured by western blotting. Cells were pretreated with vehicle or GS12021 (20 μM for 1 h) and stimulated with LPS for the time periods indicated. Quantification results are shown in the right panel. *<i>P</i> < 0.05 <i>versus</i> CON; ^#<i>P</i> < 0.05, ^##<i>P</i> <0.01 <i>versus</i> LPS alone. (B) Cells were treated with GS12021 (20 μM) for the indicated time periods and western blotting was performed with specific antibodies against p-ACC, p-AMPKα, and SIRT1. β-tubulin was used as a loading control. (C) Concentration-dependent effect of GS12021 on AMPK activation and SIRT1 expression. Cells were treated with different concentrations of GS12021 for 12 h, and western blotting analyses were performed.