Effect of LMWHs on cellular viability.

<p><b>(A)</b> LDH activity of PBMC supernatants from asthmatic subjects (n = 5) expressed as the percentage of maximum LDH release following 72 hours of incubation in the presence of either PHA, enoxaparin/dalteparin alone or enoxaparin/dalteparin co-incubated with PHA. Error bars indicate standard deviation. ***<i>p</i><0.001 compared to unstimulated cells only control. <b>(B)</b> Effect of enoxaparin and dalteparin on the viability of PBMCs obtained from asthmatic subjects (n = 5) after pre-treatment of cells with either PHA, enoxaparin/dalteparin alone or enoxaparin/dalteparin co-incubated with PHA. Viability was measured using trypan blue dye exclusion test 72 hours after incubation with drugs and is presented as the percentage of viable cells. Error bars indicate standard deviation. *<i>p</i><0.05, **<i>p</i><0.01 and ***<i>p</i><0.001 compared to either unstimulated cells only or PHA-stimulated control. <b>(C)</b> The effects of enoxaparin and dalteparin on PHA-induced proliferation of PBMCs obtained from asthmatic subjects (n = 5) measured 72 hours after incubation with enoxaparin/dalteparin alone or enoxaparin/dalteparin co-incubated with PHA. Error bars indicate standard deviation. **<i>p</i><0.01 and ***<i>p</i><0.001 compared to either unstimulated cells only or PHA-stimulated control.