The selective GR modulator compound A does not affect cell viability of PBMCs.

<p><i>(A)</i> PBMC cells were treated with compound A (CpdA) (0.1μM, 1μM or 10μM) for 1h, followed by a 24h incubation with SEB (0.5μg/ml). Cell culture media were analyzed for the presence of LDH. Averaged results of 10 patient samples are shown ± SEM. Statistical analysis was performed using a Friedman test to compare all samples. ns, not significant <i>(B)</i> Six selected samples were diluted 1:2 and 1:4 and measured for LDH activity. Results of the undiluted sample were set as 1 and results of diluted samples were recalculated accordingly to obtain the ‘calculated dilution factor’. The resulting calculated dilution factors are presented as a box and whiskers plot and graphed against the theoretically expected value, i.e. the dilution factor on the X-axis. <i>(C)</i> PBMCs of 6 patients were exposed to solvent, methylprednisolone (MP) (10μM) or compound A (CpdA) (10μM). PBMCs of 4 patients were exposed to staurosporine (STS) (10μM) for 24 h, as a positive control. Cell apoptosis and cell death was analyzed using flow cytometric analysis gated on the lymphocytes, of annexin V binding and propidium iodide staining, respectively. Averaged results are shown ± SEM. Statistical analysis was performed using a two-way ANOVA with Bonferroni post-tests to analyze the significance of treatments versus the solvent control. ns, not significant; **, <i>P</i><0.01; ***, <i>P</i><0.001.