GBM8401 SP cells express higher level of stemness CD markers than the parental and non-SP (NSP) cells.

<p>GBM8401 SP and non-SP cells were sorted and then incubated with specific anti-CD marker antibodies or with isotype control for 1 h, respectively. After wash with PBS and centrifugation, cells were re-suspended with PBS and analyzed by flow cytometry. The histograms of flow cytometry analysis of CD133 (A), CD24 (B), CD26 (C), and CD44 (D) were shown. The expressions of the above stemness markers in SP cells were significantly higher than those in non-SP cells. However, the expressions of other markers, such as CD29, CD90, and SSEA-4, were not apparently different (data not shown).