Flow cytometry and gene expression analysis.
- Martha G. Castillo (14486274)
- Tomás M. Peralta (14486276)
- Paola Locatelli (5492981)
- Candela Velazquez (14486278)
- Yamila Herrero (14486280)
- Alberto J. Crottogini (14486282)
- Fernanda D. Olea (14486284)
- Luis A. Cuniberti (14486286)
Publication date
January 2023
DOI Publisher
Public Library of Science (PLoS)Abstract
Labeling of A: AT-Muse cells and B: ASCs with antibodies anti- SSEA3, CD45, CD44, CD166 and controls. C: The % of SSEA3+ cells was higher in the AT-Muse group than in the ASCs group. D: Immunofluorescence image confirming the labeling of SSEA3 (green) and DAPI (blue) in AT-Muse cells at P4 in vitro. Scale bar = 100 μm. E: AT-Muse cells were CD166+, CD44+ and CD45- confirming their immunophenotype. F-I: The AT-Muse group showed higher expression of OCT4, NANOG and S1PR2 than the ASCs group. Results are means ± SD. *p<0.05, Mann-Whitney U test.</p
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