Characterization of MSCs derived from BM, CB and PB.

<p>(A) Morphology of cultured BM-MSCs, CB-MSCs and PB-MSCs. MSCs showed fibroblastic-like morphology. Magnification: ×40, ×100. (B) RT-PCR analyses for genes expressed in MSCs. Oct4 and Nanog are undifferentiated stem cell markers. CD90 is a positive marker of MSCs. CD133 is a negative marker of MSCs. (C) Flow cytometric analysis of cell surface markers of MSC from BM, CB and PB. MSCs expressed CD90, and CD44 but not CD34, CD45, and CD133. CD34, CD45, and CD133 are negative markers, and CD90, and CD44 are positive markers.