Immunization Schedule.

<p>For: <b>A.</b> Mice, animals were immunized four times at 30 days interval with 20 μm of <i>Pvs</i>48/45 formulated with Freund Adjuvant. Blood samples were taken on days 0, 30, 60 and 90 for ELISA testing and on day 120 for functional and biological assessing. <b>B.</b> Monkeys, two experimental animals were immunized with 50 μg of <i>Pvs</i>48/45, and one control group with PBS emulsified in 1:1 with Montanide ISA 51 Adjuvant. Protein was administrated subcutaneously in the back at 20 days intervals, blood samples were taken before each immunization, 15 days after the third immunization and then at 30 days intervals until day 150. EG: Experimental group; CG: Control group; CFA: Freund Complete Adjuvant; IFA: Freund Incomplete Adjuvant; EM: Experimental Monkeys, CM: Control Monkey, ISA 51: Montanide ISA 51 Adjuvant, Prot. <i>Pvs</i>48/45 protein.