Immunogenicity of C-S8p260 in mice. C57BL/6 mice were inoculated in the FP with either 10³ or 10⁷ PFUs of C-S8p260, or PBS.

<p>At 90 days post-immunization, mice were challenged with 10⁴ PFUs of FMDV C-S8c1 (procedures are described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0010414#s4" target="_blank">Materials and Methods</a>). A. Survival curves after immunization and after challenge (indicated in the abscissa, with arrow in the bottom panel). Continuous line: animals immunized with C-S8p260; discontinuous line: animals inoculated with PBS. B. IgG titers determined by ELISA at different days post-immunization and after challenge (abscissa and bottom panel). C. IgG titers in serum of mice immunized with BEI-inactivated C-S8p260. The day of the challenge with 10⁴ PFUs of C-S8c1 is indicated with an arrow; 16 animals were inoculated in each group. D. Survival of mice inoculated with C-S8p260p3d and C-S8c1; 21 mice were inoculated with 10⁷ PFUs C-S8p260p3d (squares), and 10 mice with 10³ PFUs of C-S8c1 (diamonds).