Immunization schedules.

<p>A. Guinea pig immunization. Twenty seven guinea pigs were divided into 5 groups (n=6 for rLaSota/gp160, rLaSota/gp140L, rLaSota/gp140S, rLaSota/gp120 groups; n=3 for rLaSota group). Animals in each group were immunized with two doses of each recombinant virus on days 0 and 14 by i.n. route of administration. Each dose consisted of 300 µl (150 µl in each nostril) of allantoic fluid containing 10⁶ PFU/ml of virus. Blood, vaginal washes and fecal samples were collected on days 0, 7, 14, 21, 28, 42, 56, 70 and 90. All animals were sacrificed on day 90. B. Mice immunization. Thirty mice were divided in to 5 groups (n=6/group). Animals in each group were immunized with two doses of virus on days 0 and 14 by the i.n. route of administration. Each dose consisted of 50 µl (25 µl in each nostril) of allantoic fluid containing 10⁵ PFU/ml of virus. All animals were sacrificed on day 56 and splenocytes were collected.