Moderate PERK activation promoted Daoy cell migration and invasion.

<p>(A) Wound healing assay showed that AP20187 (0.001–0.01 nM) treatment significantly increased the percentage of filled wound area of Fv2E-PERK1 cells. (B, C) Results of matrigel transwell assay. Daoy or Fv2E-PERK1 cells were seeded in the top well of a matrigel-coated chamber. After 16 h, the invaded cells at the lower surface of the chamber were stained with crystal violet and photographed (C). The stained cells were dissolved in 10% acetic acid and the absorbance was measured at 561 nm (B). AP20187 (0.001–0.01 nM) treatment significantly increased the invaded Fv2E-PERK1 cell numbers at the lower surface of the chamber. The experiments were repeated at least three times. Error bars represent SD, *<i>P</i> < 0.05.