Invasive behavior of DU-145 expressing gal-7^wt or gal-7^R74S.

<p>(<b>A</b>) Matrigel invasion assay of DU-145 stable transfectants. Cells were seeded into serum-free medium in the upper chamber, and serum was added to the lower chamber. After 16 h, cells that migrated through the Matrigel and the 8 μm-pore membrane were stained and counted. The results represent the number of cells per microscopic field. (<b>B</b>) Plots of 30 cells/sample tracked by live cell imaging during the scratch wound healing test. DU-145 cells were seeded onto a 6-well glass-bottom culture plate. A scratch was made, and images were captured every 10 min for 2 h. Quantifications of the (<b>C</b>) velocity, (<b>D</b>) accumulated distance, (<b>E</b>) Euclidean distance and (<b>F</b>) directionality are shown. Error bars represent the SEM. All results represent three independent experiments, including a minimum of two independent DU-145 clones. **P ≤ 0.01 and ***P ≤ 0.001.