Infection of wildtype mice with <i>C</i>. <i>rodentium</i> activates Stat3 in the intestinal epithelium.

<p>A) Luminescent <i>C</i>. <i>rodentium</i> was visualized and quantified in live wildtype mice at different time points after infection using IVIS. Exemplary pictures on day three (left) and day seven (right) are shown. Data show mean values + SEM (n = 4 mice). B) Representative colonoscopy pictures demonstrate absence of colitis in unchallenged mice (top) and inflammation in the colon of <i>C</i>. <i>rodentium</i> infected mice (bottom, day six after infection). C) Transcription of Stat3-inducing cytokines in the colon of <i>C</i>. <i>rodentium</i> infected wildtype mice (n = 4) and unchallenged wildtype mice (n = 5). Gene transcription is normalized to <i>Hprt</i>. Data show mean values + SEM. D) Colon cross-sections of wildtype mice were stained for phosphorylated Stat3 (pStat3, red) at different time points of <i>C</i>. <i>rodentium</i> infection. Nuclei are shown in blue. Scale bars: 100 μm. The experiment was performed four times with similar results.