Immunocytochemical characterization of neurospheres generated from sorted YFP+ve neural crest-derived cells.

<p>YFP+ve neurospheres contain cells positive for neural crest and neural stem/progenitor markers p75 (A), Sox10 (B) and Nestin (C), the neuronal marker TuJ1 (D), glial markers GFAP (E) and S100 (F), and the proliferation markers PH3 (G) and Ki67 (H, arrows). YFP+ve neurospheres do not contain cells expressing the ICC marker c-Kit (I) or the smooth muscle marker, SMA (J). All neurospheres show YFP+ve cells in green, corresponding markers in red and DAPI (nuclei) in blue. Scale bar = 50μm.