Neurospheres derived from postnatal human gut cultures are heterogeneous in composition.

<p>(A) Bright field image of neurosphere derived from human postnatal gut cultured for 2 weeks. Neurospheres contain neural crest-derived cells as shown by expression of the neural crest cell marker p75 (B) and the neuronal marker TuJ1 (C). Neurospheres also contained non-neural crest cell derivatives such as fibroblast-like cells expressing PDGFRα (D), and muscle cells expressing SMA (E). Proliferating cells are labelled with Ki67 (F). Following FACS sorting based on selection with p75^NTR, neurospheres were generated exclusively from p75-ve (G) and p75+ve cells (H) similar in shape and appearance. DAPI (blue) stains nuclei in B-F. Scale bar = 50μm.