<p>Neurospheres derived from YFP-ve cells do not contain cells expressing neural crest cell markers p75 (A), Sox10 (B), neuronal marker TuJ1 (D) or glial markers GFAP (E) or S100 (F). However they do contain cells expressing Nestin (C), proliferation markers PH3 (G, arrows), Ki67 (H, arrows), ICC marker c-Kit (I) and smooth muscle marker SMA (J). All markers in red and DAPI (nuclei) in blue. Scale bar = 50μm.</p
<p>(<b>A</b>) Morphology of PBT003, PBT008, PBT022 and PBT030 neurospheres in stem cell culture medi...
<p>Spheres derived from the various regions were morphologically similar (A), Neurospheres initiatin...
Human neurospheres are free-floating spherical clusters generated from a single neural stem cell and...
<p>YFP+ve neurospheres contain cells positive for neural crest and neural stem/progenitor markers p7...
<p>(A) Bright field image of neurosphere derived from human postnatal gut cultured for 2 weeks. Neur...
<p><b>a</b>) Phase microscopy of a single neurosphere derived from a P10 FT culture after 10 days <i...
<p>A) Phase contrast photomicrograph of neurosphere-like bodies after 10 days culture. Arrow points ...
<p><b>A.</b> Flow cytometry profile showing the YFP+ cell population (green) separate from the enter...
<p><b>a</b>) Differentiated cells derived from a single neurosphere double stained for the NPC marke...
Neural stem cells (NSCs) are important multipotent adult stem cells capable of self-renewal and diff...
<p><b>A.</b> Neurospheres formed on day 8 in culture are nestin-GFP+ and Tuj1+. Nuclei were stained ...
<p><b>A:</b> Phase contrast image (20x magnification, scale bar = 90 uM) of hMSC Phase A neurosphere...
<p>A, Phase-contrast image of passage 1–3 neurospheres formed by SFM1022 cells and WT-iPSCs. B, Immu...
<p>(A) FACS isolated GFP+/tdTomato+ cells from <i>Gli1<sup>CreER/+</sup>;hGFAP-GFP;R26<sup>tdTomato/...
<p>Wild-type (A) or 3KO (B) neurospheres made from E14.5 cortex were differentiated for five days an...
<p>(<b>A</b>) Morphology of PBT003, PBT008, PBT022 and PBT030 neurospheres in stem cell culture medi...
<p>Spheres derived from the various regions were morphologically similar (A), Neurospheres initiatin...
Human neurospheres are free-floating spherical clusters generated from a single neural stem cell and...
<p>YFP+ve neurospheres contain cells positive for neural crest and neural stem/progenitor markers p7...
<p>(A) Bright field image of neurosphere derived from human postnatal gut cultured for 2 weeks. Neur...
<p><b>a</b>) Phase microscopy of a single neurosphere derived from a P10 FT culture after 10 days <i...
<p>A) Phase contrast photomicrograph of neurosphere-like bodies after 10 days culture. Arrow points ...
<p><b>A.</b> Flow cytometry profile showing the YFP+ cell population (green) separate from the enter...
<p><b>a</b>) Differentiated cells derived from a single neurosphere double stained for the NPC marke...
Neural stem cells (NSCs) are important multipotent adult stem cells capable of self-renewal and diff...
<p><b>A.</b> Neurospheres formed on day 8 in culture are nestin-GFP+ and Tuj1+. Nuclei were stained ...
<p><b>A:</b> Phase contrast image (20x magnification, scale bar = 90 uM) of hMSC Phase A neurosphere...
<p>A, Phase-contrast image of passage 1–3 neurospheres formed by SFM1022 cells and WT-iPSCs. B, Immu...
<p>(A) FACS isolated GFP+/tdTomato+ cells from <i>Gli1<sup>CreER/+</sup>;hGFAP-GFP;R26<sup>tdTomato/...
<p>Wild-type (A) or 3KO (B) neurospheres made from E14.5 cortex were differentiated for five days an...
<p>(<b>A</b>) Morphology of PBT003, PBT008, PBT022 and PBT030 neurospheres in stem cell culture medi...
<p>Spheres derived from the various regions were morphologically similar (A), Neurospheres initiatin...
Human neurospheres are free-floating spherical clusters generated from a single neural stem cell and...