Con-G repairs MCAO-induced morphological changes.

<p>(A-L) Representative images of H & E stained coronal brain sections showing perturbed brain architecture of the ipsilateral side at 4 hr and 26 hr for non-con-G treated (A, 4 hr; G, 26 hr) and con-G treated rats (D, 4 hr; J, 26 hr), characterized by perineuronal vacuolation (black arrows) and shrunken neurons (block arrows), which is less dramatic at 4 hr (A) than at 26 hr (G). MCAO-induced pathology in the stroke area is similar in con-G treated brains at 4 hr (D) and 26 hr (J) compared to non-con-G treated brains. (B, E, H, K) show the normal brain architecture of the contralateral region of non-conG treated and con-G-treated rats at 4 hr and 26 hr after stroke induction. (C) The peri-infarct region at 4 hr and (I) 26 hr of con-G-treated and non-treated stroked rats with perineuronal vacuolation and degenerating neurons (<) observed. However, the peri-infarct region of con-G-treated rat brains shows repair of the brain cytoarchitecture at 4 hr (F). The perineuronal vacuolation, while present, is not as pronounced (arrows), with fewer degenerated neurons (<). (L) 26 hr con-G-treated rat brains showed enhanced cytorepair with unpronounced perineuronal vacuolation (black arrow) and diminished eosinophilic neurons (black circle). Total magnification, 200X. Bar scale, 60 μm. N = 12 for S and n = 7 for S/con-G at 26 hr, and n = 4 for both S and S/con-G at 4 hr.