Oligodendrocyte maturation <i>in vitro</i> using WT-derived NS.

<p>A) Immunocytochemistry of NS cultures pre-treated during proliferation with bFGF/EGF or bFGF/PDGF-BB, and then exposed to differentiating conditions; No growth factors (CTL), Tri-iodothyronine (T3), Insulin-like Growth Factor 1 (IGF-1) and Fetal Calf Serum (FCS). B) Comparison of the proportion of MBP⁺ cells detected after differentiation between bFGF/EGF and bFGF/PDGF-BB pre-treated NS. C) Quantitation of the MBP⁺ area/nuclei ratio after differentiation in two independent cultures. Data in B and C was analyzed with a Two-way ANOVA and Bonferroni post-test to compare the effect of NS pre-treatment during proliferation (*) or the differentiation treatment compared to its CTL (#). D) Fold change of the MBP⁺ area/nuclei in bFGF/PDGF-BB compared to bFGF/EGF cultures. The bars in B, C and D represent the mean + SD. The scale bar in A (i) represents 100 μm for (i) and (ii), while the scale bar in A (iii) represents 100 μm for (iii) to (x). * = p < 0.05. ** = p < 0.01 and # = p < 0.05. DIFF.; Differentiation.