Role of vitronectin in oligodendrocyte differentiation.

<p><b>A: Vitronectin increased oligodendrocyte differentiation.</b> A1–2. Photomicrographs of P0 cerebellar slices labeled with antibodies against MBP at 7DIV. The density of MBP+ elements in the control slice P0–7 DIV (A1) was lower than that in the slice grown in the presence of vitronectin (P0–7 DIV+VN, A2). A3. Quantification of MBP immunostaining in P0–7DIV immature slices. * P<0.05 (Mann-Whitney test). Ctrl (control slices), and VN (P0–7 DIV grown in the presence of vitronectin). <b>B: Immunoblots revealing the presence of vitronectin.</b> Immunoblot of vitronection (VN) in total lysates from P0–10 DIV cerebellar slices: control (Ctrl) or 2 DIV axotomy (Axt) or BrdU treated (BrdU) slices. Note the increase of vitronectin in BrdU treated slices in comparison to control and axotomized slices. The clathrin heavy chain (CHC) has been used as a loading control. <b>C:</b> Photomicrographs of P0 cerebellar slices labeled with antibodies against MBP at 7 DIV. C1. P0–7 DIV slice grown in the presence of CM (conditioned medium, as explained in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0049015#pone-0049015-g002" target="_blank">Fig. 2</a>). C2. P0–7 DIV slice grown in the presence of CM and an antibody against VN. C3. P0–7 DIV slice grown in the presence of CM and a control antibody. C4. Quantification of the density of MBP immunostaining in P0–7 DIV immature slices grown under various conditions: Control P0–7 DIV slices (Ctrl). In the presence of a blocking antibody against vitronectin (+ anti-VN). In the presence of a control antibody (+Ctrl antibody). With medium conditioned by P0 7–14 DIV control mature slices (CM). With medium conditioned by P0 7–14 DIV mature slices and a blocking antibody against vitronectin (CM+anti-VN). With medium conditioned by P0 7–14 DIV mature slices and a control antibody (CM+Ctrl antibody). *** P<0.001 versus the control (Mann-Whitney tests). Scale bar in A1 is 418 µm in A1–2 and 375 µm in C1–3.