Matrigel cultures of OPCs.

<p>A) Oligodendrocyte progenitor cell matrigel culture where a PBS-soaked bead was placed 0.5 mm away from the cell cluster. Image taken at 40X magnification. B–C) OPCs where a cluster of cells migrating towards a FGF8-soaked bead can be observed. D) Histogram depicting the oligodendrocyte progenitor cell proximal/distal rate in control cultures and where FGF8 was included. *p<0.01 (paired t-test, n = 4). (E–G) Matrigel cultures where on one side a FGF8-soaked bead was placed and on the other side a FGF8 inhibitor. (F, G) Close-up images of the areas near the inhibitor-soaked bead (F) or near the FGF8-soaked bead (G). H) OPCs where a FGF8-soaked bead was placed and on the other side a PBS-soaked bead, as control of the inhibitor. Scale bar indicates 500 µm for (A–C, D, H) and 200 µm for (F, G). All images were taken 48 hours after culture.