Salivary nucleosides induce expression of a regulatory T cell profile in effector T cells.

<p>Ear cells from mice infected with <i>Leishmania amazonensis</i> in the presence of ADO+AMP (■) or PBS (□) were harvested at 11 weeks post infection. <i>A</i>, Lymphocytes from the ears were phenotyped by flow cytometry with FITC-conjugated anti-CD3, PerCP-conjugated anti-CD4, and PE-Cy7-conjugated anti-CD25 Lymphocytes were selected with CD4⁺CD25⁺ (<i>B</i>) or CD4⁺CD25^- (<i>C</i>) gates, and these populations were subsequently analyzed for expression of FoxP3, CD39, CD73, and CD103. CD4⁺CD25^-lymphocytes (2 × 10⁶ cells/ml) were isolated from draining lymph nodes of PBS- (□) or ADO+AMP- (■) infected mice at 8 weeks post infection. The lymphocytes were re-stimulated in vitro with αCD3 (2 μg/ml) plus αCD28 (1 μg/ml) for 96 h. CD4⁺CD25⁺ cells were added to some of the CD4⁺CD25^-culture wells. <i>D</i>, Suppressive activity was determined by ELISA to measure IL-10 secretion in the culture supernatants. Data are expressed as the means ± SEM and are representative of two independent experiments that were each performed with four mice per group (<i>n</i> = 4). ^#, <i>p</i><0.05 relative to the PBS-treated group.