IL-22 is induced during leishmania infections.

<p>C57BL/6 mice were intradermally infected with 2 x 10⁶<i>L</i>. <i>major</i> promastigotes metacyclics in the ear. Cells from the draining lymph nodes of infected mice were isolated and cultured for 72 hours with media or leishmania antigen. Supernatants were collected and <b>(A)</b> IFN-γ <b>(B)</b> IL-17, and <b>(C)</b> IL-22 release was measured by ELISA. <b>(D)</b> C57BL/6 mice were intradermally infected with 2 x 10⁶<i>L</i>. <i>major</i> promastigotes metacyclics in the ear and two days later treated with anti-CD4. Mice were euthanized on day 3 and cells from the draining lymph nodes were isolated and cultured for 72 hours with media or leishmania antigen to analyze IL-22 production by ELISA. <b>(E)</b> PBMCs from healthy subjects and <i>L</i>. <i>braziliensis</i> infected patients were cultured for 72 hours with media or <i>L</i>. <i>braziliensis</i> antigen. Supernatants were collected and analyzed for IL-22 release by ELISA. Data are representative of at least 3 independent experiments, with 3–5 mice per group. Error bars indicate mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.