Neto-β is a novel Neto isoform at the <i>Drosophila</i> NMJ.

<p>(A) Diagram of the <i>Drosophila neto</i> locus. Two isoforms are generated by alternative splicing, Neto-α and Neto-β. They have different cytoplasmic domains, but share highly conserved domains, CUB (complement <u>C</u>1r/C1s, <u>U</u>egf, <u>B</u>MP1), LDLa (LDL receptor class a) and transmembrane (TM), with Neto proteins from vertebrates and <i>C</i>. <i>elegans</i>. (B) Confocal images of third instar larvae NMJ4 from <i>neto</i>^<i>null</i> animals rescued with <i>neto</i>-<i>α</i> (<i>neto</i>^<i>36</i>;<i>G14>neto</i>^<i>α</i>), <i>neto</i>-<i>β</i> (<i>neto</i>^<i>36</i>;<i>G14>neto</i>^β), and a <i>neto</i> transgene lacking any intracellular part, <i>neto</i>Δ^intra<i>(neto</i>^<i>36</i>;<i>G14>neto</i>Δ^intra). These Neto variants can rescue the viability and NMJ development in <i>neto</i>^<i>null</i> animals. The Neto-ex signals mark all NMJs, but the Neto-β antibodies specifically label control and <i>neto-β</i> rescued NMJs. (C-D) Confocal images of late embryos ventral muscle fields (indicated in white) labeled for (C) Neto-ex (red), Neto-β (green), HRP (blue) and (D) GluRIIA (red), Neto-β (green), HRP (blue) indicating the presence of Neto-β in the early stages of the larvae development. Genotypes: control (<i>w</i>^<i>1118</i>); <i>neto</i>^<i>36</i>;<i>G14>neto</i>^<i>α</i> (<i>neto</i>^<i>36</i><i>/Y; G14-Gal4/UAS-neto-A9</i>); <i>neto</i>^<i>36</i>;<i>G14>neto</i>^β (<i>neto</i>^<i>36</i>, <i>UAS-neto-B6/Y; G14-Gal4/+</i>); <i>neto</i>^<i>36</i>;<i>G14>neto</i>Δ^intra(<i>neto</i>^<i>36</i><i>/Y; G14-Gal4/ UAS-neto</i>Δ^intra<i>-H4</i>). Scale bars: (B) 20 μm (C-D) 10 μm; 5 μm in details.