Neto prodomain affects synapse structure and function.

<p>(A) Electron micrographs of synaptic boutons from control and PM-Neto rescued (<i>G14>PM-neto-GFP-D2</i>) third instar larvae. Details are shown in lower panels and a larger field in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004988#pgen.1004988.s005" target="_blank">S5 Fig.</a>. The SSR, active zone (AZ), and PSDs (brackets) are indicated. SSR was densely packed around control type Ib boutons, but was severely disrupted at PM-Neto rescued boutons, which also showed loss of PSDs, floating T-bars (dark arrowheads), and enlarged synaptic vesicles (white arrowheads). (B-F) The numbers of synaptic vesicles, T-bar sizes, and PSDs length were compared. The numbers of boutons (B and D) or active zones (C, E and F) examined are indicated in each bar. (G-L) Electrophysiological recordings at PM-Neto rescued or overexpressed NMJ. Representative traces of spontaneous (G) and evoked (J) neurotransmitter release recorded from muscle 6 of indicated genotypes. The frequency and amplitude of mini EJPs were decreased in PM-Neto rescued NMJ (H-I). Evoked EJPs were largely normal (corrected values shown in K) due to presynaptic compensation (L). The numbers of NMJs examined are indicated in each bar. Error bars indicate SEM. *; <i>p</i><0.001, **; <i>p</i><0.01, ***; <i>p</i><0.05.