Variant allele frequencies of relevant mutations in sequenced pools of mating offspring under no drug, intermediate, and high-level selection for each drug.

<p>^a The listed resistant strain was mated with the isogenic sensitive strain of the opposite mating type. (e.g. AFB62I11 (ItrR) was mated with AFB62F9)</p><p>^b 1: represents a variant allele frequency of 1 (i.e. only the mutant SNP was found). 0.5: represents a 1:1 ratio of the reference and mutant alleles. Ref: signifies only the reference allele was detected in the pool.</p><p>^c Mutations associated with the resistance phenotype because they followed the expected pattern are highlighted in bold. All blank cells represent the reference allele and were left blank because those mutations were not relevant in a given cross.</p><p>Variant allele frequencies of relevant mutations in sequenced pools of mating offspring under no drug, intermediate, and high-level selection for each drug.