Distinguishing germline variants from somatic variants.

<p>(A) For each SNP, the non-reference allele frequencies for samples (out of seven) with smallest (black) and largest (gray) such frequencies are shown. In all samples, these frequencies do not deviate substantially from the germline frequencies. All SNPs in all samples have minor allele frequencies near the expected 50% or 100% and are therefore clearly and consistently distinguishable as either homozygotes (leftmost three) or heterozygotes (the remainder). (B) In contrast, the somatic variants display much wider ranges in allele frequencies across samples. Note that the absence of black bars is indicative complete absence of the mutation in some samples.