3D Structured Illumination Microscopy images of U2OS cells expressing DG and DG^T190M fused to EGFP.

<p>DG-EGFP (A) and DG^<b>T190M</b>-EGFP (B) are both localized specifically at the plasma membrane and accumulate in the cytoplasm. The columns show the individual images for DAPI (blue), EGFP (green), Cellmask Plasma Membrane Orange (red) and the resulting RGB overlay. The first row of images is the z stack maximum intensity projection of the 3D recording of the cell. The two subsequent rows of images are at a specific z position in the stack (XY) and X position slice through the stack (XZ). The white bar represents 10μm. Immunolabelling of Myc-tagged Dystroglycan (C) shows a specific signal at the plasma membrane. The individual maximum intensity z-stack projection images for the EGFP, Cellmask Orange and anti Myc channels are shown as well as an overlay. Four by four μm magnifications of the corresponding white dashed boxes depict the Myc-tag signal at the outside of the plasma membrane (filopodia). The antibody stain appears nicely shifted toward the outside of the plasma membrane, while GFP and Cellmask orange are overlapping.