Systemic infection of <i>A. thaliana</i>.

<p>Northern blot analysis of RNA extracted from upper, non-inoculated leaves of <i>A</i>. <i>thaliana</i> plants infiltrated with the wild-type TuYV (WT) or one of the TuYV-3a mutants, TuYV-3aAUG (AUG), TuYV-3aAGC (AGC), TuYV-3a2stop (2stop) or TuYV-3aFLAG (FLAG). All plants were analyzed but only three samples representative for the TuYV-WT and each mutant are shown, with at least one sample of an infected plant, confirmed by RT-PCR. Mock, mock-inoculated control; gRNA, genomic RNA; sgRNA1, subgenomic RNA1, LC, loading control of ethidium bromide stained ribosomal RNA.