Quantitative RT-PCR analyses of <i>GFP</i> and <i>PnPMA1</i> expression in <i>Phytophthora parasitica</i>.

<p>(<b>A</b>) <i>GFP</i> (a) and <i>PnPMA1</i> (b) abundance in <i>P. parasitica</i> strain 1-2–1-1 stably expressing ER-rendered GFP in infected detached leaves. (<b>B</b>) Quantification of <i>GFP</i> (a-c) and <i>PnPMA1</i> (d–f) in 1-1–2-1 in infected roots 12 h, 24 h and 48 h post inoculation, respectively. WT, wild-type <i>A. thaliana</i>; CK, <i>A. thaliana</i> transformed with pART27; 1, 2 and 3 are three independent transgenic <i>A. thaliana</i> lines that accumulated <i>GFP</i> or <i>PnPMA1</i> siRNAs (the same set of lines as shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0028114#pone-0028114-g002" target="_blank">Figure 2</a>). <i>P. parasitica WS041</i> was used as a reference gene for quantification and normalization of <i>P. parasitica</i> gene expression. The data presented are relative gene expression calculated after being normalized to <i>WS041</i> with the 2^−ΔΔct method. The data represent the means of three replicates. Bars represent the standard errors of three independent measurements. Statistical significance of the target genes silencing effect was performed by Student's <i>t</i> test using <i>R</i> (one-tailed, transgenic lines versus wild-type).