Deletions of the TNV-A^C coat protein gene and plant inoculations.

<p>(A) Genomic organization of wtTNV-A^C (pMTC27) ORFs are shown as gray boxes. The six mutant constructs are shown below and the associated dashed lines indicate deleted nucleotide fragments whose positions within TNV RNA are identified by numbers within the deleted regions. (B) The phenotype of <i>C. amaranticolor</i> leaves inoculated with mutant RNAs were photographed at 4 dpi. Viral RNAs used for inoculations are shown above each photo, including a mock inoculation with buffer alone on the upper left leaf. (C) Northern blot detection of wtTNV-A^C and deletion mutants viral RNAs isolated from inoculated <i>C. amaranticolor</i> leaves. A cDNA fragment derived from the TNV-A^C 3′ UTR was labelled with a ³²P labeled probe to assess viral RNA accumulation. The positions of TNV-A^C RNA species are indicated on the left side of the gel photographs, and plant ribosomal RNAs (rRNA) used as loading controls are shown in the bottom panel.