NaSal failed to increase excitability of 5-HTergic neurons in the absence of the GABAergic inhibition in the DRN of the ChR2 transgenic mouse.

<p>(A) Sample recordings (left panel) and group data (right panel, two RM-ANOVA and paired Student’s <i>t</i>-test) showing that NaSal did not increase the current-evoked firing of 5-HTergic neurons with the GABAergic synaptic transmission blocked by 100 μM PTX. (B) Sample recordings from a 5-HTergic neuron showing that NaSal decreased voltage responses to a series of hyperpolarizing currents (-10 to -60 pA, -10 pA/step, duration: 500 ms). The RMPs are indicated. (C) Group data showing that NaSal hyperpolarized the RMP and decreased the <i>R</i>_in in 5-HTergic neurons (paired Student’s <i>t</i>-test). Sample sizes are indicated in inset. Vertical line bars represent one standard error. *<i>P <</i>0.05 relative to control.